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[目的]探讨增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白在膀胱癌中的表达情况,并分析其与临床病理特征及复发的关系。[方法]148例膀胱移行细胞癌患者标本,25例癌旁正常组织标本作为对照,采用免疫组化SP法检测PCNA蛋白的表达。[结果]癌旁正常组织中PCNA蛋白的阳性表达水平明显低于肿瘤组织(16.0%vs50.7%,P<0.001)。PCNA蛋白的表达与膀胱癌的分级、分期、复发有关(P<0.05),与患者的年龄、性别、肿瘤数目及肿瘤大小无关(P>0.05)。本组104例浅表性膀胱癌(Ta～T1)随访2～95个月,其中复发64例,复发组与未复发组PCNA蛋白的阳性表达率分别为56.3%和15.0%(P=0.001)。[结论]PCNA蛋白在膀胱癌中高表达,其高表达提示预后不良。
[Objective] To investigate the expression of proliferating cell nuclear antigen (PCNA) in bladder cancer and to analyze its relationship with clinicopathological features and recurrence. [Method] 148 cases of transitional cell carcinoma of the bladder and 25 cases of adjacent normal tissues were used as control. The expression of PCNA protein was detected by immunohistochemical SP method. [Results] The positive expression of PCNA protein in adjacent normal tissues was significantly lower than that in tumor tissues (16.0% vs 50.7%, P <0.001). The expression of PCNA protein was correlated with the grade, stage and recurrence of bladder cancer (P <0.05), but not with age, sex, tumor number and tumor size (P> 0.05). The group of 104 cases of superficial bladder cancer (Ta ~ T1) were followed up for 2 ~ 95 months, of which 64 cases relapsed, the positive expression rates of PCNA protein were 56.3% and 15.0% (P = 0.001) . [Conclusion] The expression of PCNA protein in bladder cancer is high, and its high expression suggests poor prognosis.