论文部分内容阅读
目的:通过复制大鼠系统性炎症反应综合征(SIRS)模型,研究四逆汤对MODS的防治作用。方法:建立大鼠二次打击SIRS模型,SD大鼠70只,随机分成模型对照组30只,四逆汤模型治疗组30只,正常对照组10只。治疗组大鼠在一次打击后给予四逆汤灌胃,模型对照组按相同溶液体重剂量、时点灌蒸馏水。在模型建立后的1、3、5 d分别活杀大鼠。剖开胸腔,暴露心脏,快速抽取主动脉血做血气分析和有关酶学指标的检测,ELISA法测定血浆TNF-α、IL-6的含量。结果:治疗1 d组TNF-α为(35.0±14.9)ng/L,IL-6为(96.3±29.0)ng/L;5d组TNF-α为(20.9±13.7)ng/L,IL-6为(48.7±40.0)ng/L;四逆汤中药方剂能够下调实验大鼠血浆TNF-α和IL-6水平(P<0.05)。结论:四逆汤中药方剂起到抑制炎症、有效改善SIRS、MODS大鼠的各项生理生化指标、防治MODS的目的,为四逆汤防治MODS提供了实验依据。
OBJECTIVE: To study the preventive and therapeutic effects of Sini decoction on MODS by replicating the rat systemic inflammatory response syndrome (SIRS) model. METHODS: A rat model of two-stroke SIRS was established. Seventy SD rats were randomly divided into a model control group (30 control groups), a Sini Tang model treatment group (30 cases), and a normal control group (10 rats). The rats in the treatment group were given SND with gavage after one stroke. The model control group was filled with distilled water according to the body weight and time of the same solution. Rats were killed on days 1, 3, and 5 after the model was established. The thoracic cavity was dissected and the heart was exposed. Aortic blood was quickly extracted for blood gas analysis and related enzymatic indicators. Plasma TNF-α and IL-6 levels were determined by ELISA. Results: The TNF-α was (35.0±14.9) ng/L in the first day of treatment, while the IL-6 was (96.3±29.0) ng/L. The TNF-α in the 5d group was (20.9±13.7) ng/L, IL-6. It was (48.7±40.0)ng/L; Sini Decoction can down-regulate the levels of plasma TNF-α and IL-6 in experimental rats (P<0.05). Conclusion: Sini Decoction prescription can play an important role in inhibiting inflammation, effectively improving the physiological and biochemical indexes of SIRS and MODS rats, and preventing and treating MODS. It provides experimental basis for the prevention and treatment of MODS by Sini decoction.