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利用共表达网络分析法(WGCNA)筛选出合浦珠母贝外套膜在高温胁迫下影响显著的1个响应基因模块和海水酸化胁迫下的5个响应基因模块,并对模块中包含的基因进行功能富集;分析贝壳蛋白与模块其他基因的关联性,构建矿化基因的应激网络。高温显著响应模块基因富集结果显示细胞骨架蛋白发生了显著的变化,如ARHGEF4、SAP和myosin。酸化应答模块共5个,响应的基因包括呼吸相关的血红素A合成酶、芳香族氨基酸代谢相关的酶、钙粘蛋白和血小板反应蛋白等。另外,高温应答模块聚集了最多矿化基因,包括MSI、Tyrosinase、Fibronectin-like protein等重要的矿化基因,说明这些矿化基因对温度较为敏感。构建基因共表达网络的结果显示钙离子结合蛋白、细胞骨架蛋白、蛋白质糖基化相关的基因关系密切。
A co-expression network analysis (WGCNA) was used to screen out one response gene module and five response gene modules under the stress of seawater stress in the mantle of Coleus coleus and to perform the function of the genes contained in the module Enrichment, analysis of the association of the shell protein with other genes in the module, and construction of a stress network of mineralized genes. Significant changes in cytoskeletal proteins were observed at high temperature and significant response to gene enrichment, such as ARHGEF4, SAP and myosin. A total of 5 acidified response modules, the response of genes including respiratory related heme A synthase, aromatic amino acid metabolism related enzymes, cadherin and thrombospondin and so on. In addition, the high temperature response module gathered the most mineralized genes, including MSI, Tyrosinase, Fibronectin-like protein and other important mineralization genes, indicating that these mineral genes are more sensitive to temperature. The results of construction of gene co-expression network showed that the genes related to calcium-binding protein, cytoskeleton protein and protein glycosylation are closely related.