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DNA拓扑异构酶是近年来分子生物学研究的热点之_,实验证明它直接与细胞增殖、DNA复制、基因表达以及抗癌药的研究密切相关。本文重点讨论了DNA拓扑异构酶的一般生物学特征及其与抗癌药研究的关系。大量实验表明该酶直接参与药物介导的DNA链断裂过程,许多药物如阿霉素、Vp16、喜树碱等都是通过影响DNA拓扑异构酶活性而发挥细胞毒作用的,目前拓扑异构酶已作为筛选抗癌药物的新靶点。
DNA topoisomerase is a hotspot in molecular biology research in recent years. Experiments have shown that it is directly related to cell proliferation, DNA replication, gene expression, and anti-cancer drug research. This article focuses on the general biological characteristics of DNA topoisomerase and its relationship with anticancer drug research. A large number of experiments have shown that the enzyme is directly involved in the drug-mediated DNA strand breakage process. Many drugs such as doxorubicin, Vp16, camptothecin, etc. exert their cytotoxic effects by affecting the activity of DNA topoisomerases, and are currently topologically heterogeneous. Enzymes have become new targets for screening anti-cancer drugs.