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目的：探讨甲状腺癌癌变的机理。方法：应用免疫组化Ｓ－Ｐ法于石蜡切片对４３例甲状腺癌、１７例腺瘤、１９例瘤旁甲状腺组织进行了ｃ－ｅｒｂＢ－２、ｐ２１、ｐ５３、Ｂｃｌ－２、ｃ－ｍｙｃ、ｐ１６癌基因产物的检测。结果：癌组织中均有不同程度１种或１种以上基因产物表达与腺瘤及瘤旁组织间均存在高度显著性差异（Ｐ＜０．０１）。ｃ－ｅｒｂＢ－２、ｐ２１表达随癌组织分化程度增高而增高。ｐ５３表达随癌组织分化程度增高而减少，与ｃ－ｅｒｂＢ－２间呈负相关（Ｐ＜０．０５）。在淋巴结转移病例中ｃ－ｅｒｂＢ－２阳性率增高明显（Ｐ＜０．０５）。结论：６种癌基因均分别参与了甲状腺癌的发生发展过程，癌变的发生可由多种基因相互作用所致。ｃ－ｅｒｂＢ－２、ｐ２１可能在甲状腺癌尤其是乳头状癌的癌变中起重要作用。癌基因产物检测为临床病理诊断、基因治疗、估计预后提供了参考指标
Objective: To explore the mechanism of carcinogenesis of thyroid cancer. METHODS: C-erbB-2, p21, p53, Bcl-2, and c-myc were performed on paraffin sections by immunohistochemical S-P method in 43 cases of thyroid cancer, 17 cases of adenoma, and 19 cases of parathyroid tissue. Detection of p16 oncogene product. RESULTS: There was a highly significant difference between the expression of one or more gene products and the adenoma and paraneoplastic tissues in cancer tissues (P<0.01). The expression of c-erbB-2 and p21 increased with the degree of differentiation of cancer tissues. The expression of p53 decreased with the degree of differentiation of cancer tissues and negatively correlated with c-erbB-2 (P<0.05). The positive rate of c-erbB-2 in lymph node metastasis was significantly higher (P<0.05). Conclusion: The six oncogenes are involved in the development of thyroid cancer. The occurrence of canceration can be caused by a variety of gene interactions. c-erbB-2, p21 may play an important role in the carcinogenesis of thyroid cancer, especially papillary carcinoma. Detection of oncogene products provides a reference for clinical pathological diagnosis, gene therapy, and prognosis estimation