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进入母体血循环的胎儿细胞有 4种。使用有效的方法从正常与异常妊娠母血中分离富集极少量的胎儿有核红细胞、滋养细胞、淋巴细胞和粒细胞。目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。分子生物学方法的引入。特别是聚合酶链反应及其衍生技术和荧光原位杂交技术提供了从母血中检测胎儿细胞敏感的方法并在无创伤性产前诊断方面得到一定的应用。
There are 4 types of fetal cells that enter the maternal blood circulation. Use effective methods to separate and collect very small amounts of fetal erythroblasts, trophoblasts, lymphocytes and granulocytes from normal and abnormal gestational maternal blood. Currently used more fluorescence activated cell sorting, magnetic activated cell sorting, immunomagnetic beads separation, density gradient centrifugation. The introduction of molecular biology methods. In particular, polymerase chain reaction and its derivatization techniques and fluorescence in situ hybridization provide a means of detecting fetal cells from maternal blood and are of some use in noninvasive prenatal diagnosis.