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本研究以PCR分子鉴定转化成功的黄瓜发状根为试材,采用悬浮培养的方法分析不同培养基类型、不同培养基组分和不同激素对黄瓜发状根生长的影响。分析显示:MS培养基中黄瓜发状根生长速度最快,其次为1/2 MS培养基,N6培养基中发状根生长的速度最慢;以MS为基础培养基,30 g/L的蔗糖为氮源,C_(NH4+):C_(NO3)-=1:2为碳源和204 mg/L KH_2PO_4为磷源时,培养基中黄瓜发状根生长量最大;以MS为基础培养基,分别添加激素IAA(Indole acetic acid)、GA3(Gibberellin)、NAA(Naphthalene acetic acid)、KT(Kinetin),其中1 mg/L IAA作用下的黄瓜发状根的增长量最大(51.0 mg),1 mg/L的NAA(Naphthalene acetic acid)处理下黄瓜发状根的增长量最少(37.8 mg/L)。以上为发状根进一步扩大培养和后期的工业化大规模生产提供一定理论依据。
In this study, PCR-based molecular identification of transformed cucumber hairy roots were used as materials to analyze the effects of different media types, different media components and different hormones on cucumber root hair growth by suspension culture. The results showed that the hairy roots of cucumber grew fastest in MS medium, followed by 1/2 MS medium, and the hairy roots grew the most slowly in N6 medium. MS-based medium with 30 g / L When the amount of C_ (NH4 +): C_ (NO3) - = 1: 2 was the carbon source and 204 mg / L KH_2PO_4 was the source of phosphorus, the maximum amount of hairy root growth was observed in the medium with MS as basal medium (IAA), GA3 (Gibberellin), NAA (Naphthalene acetic acid) and KT (Kinetin) were added respectively. The maximum amount of hairy roots was induced by 1 mg / L IAA (51.0 mg) 1 mg / L of NAA (Naphthalene acetic acid) treatment cucumber root hair growth is minimal (37.8 mg / L). The above is to provide a theoretical basis for the further expansion of hairy root culture and the large-scale industrialized production in the later period.