通过对188个微生物菌种降解胆甾醇能力的测定,发现能把胆甾醇分子完全降解成二氧化碳和水的菌种有:节细菌属、芽孢杆菌属、短杆菌属、棒状杆菌属、微杆菌属、分枝杆菌属、诺卡氏菌属、精沅细菌属、沙雷铁氏杆菌属和链霉菌属.在降解过程中如果加入抑制剂α,α’-联吡啶和亚砷酸盐可以使胆甾核分解酶受到抑制作用,使胆甾醇降解后产物得以积累而产生胆甾-1,4-二烯-3-酮和没有甾醇侧链的中间体. By measuring the ability of 188 microbial strains to degrade cholesterol, it was found that the bacteria capable of completely degrading cholesterol molecules into carbon dioxide and water are: Arthrobacter, Bacillus, Brevibacterium, Corynebacterium, Microbacterium , Mycobacterium, Nocardia, Porphyromonas, Serratia, and Streptomyces If inhibitors α, α’-bipyridyl and arsenite are added during the degradation process, Cholesterol degrading enzyme is inhibited, so that cholesterol-degrading products can be accumulated to produce cholesta-1,4-dien-3-one and no sterol side chain intermediates.