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本文报告了运用DNA重组技术和凝胶电泳技术在原发性肝细胞癌病人和慢性乙型肝炎病人的肝细胞和肿瘤细胞中发现了整合进细胞基因组的乙型肝炎病毒DNA。实验原理:(1)从肝组织中提取DNA。标本来源包括原发性肝细胞癌病人的癌组织、癌周围组织和经皮肤穿刺后所得肝组织。(2)将提取的细胞DNA进行电泳,电泳后将完整的高分子量的DNA转移并固定DBM—纤维素滤纸片上,干燥并与纯化后标记过~(32)P的克隆HBV-DNA分子进行杂交,细胞DNA中只有含有与HBV-DNA顺序严格互补的部分才能与~(32)p HBV-
This article reports the use of DNA recombination technology and gel electrophoresis to detect the integration of hepatitis B virus DNA into the cell genome in patients with primary hepatocellular carcinoma and hepatocytes and tumor cells in patients with chronic hepatitis B. Experimental principle: (1) Extract DNA from liver tissue. Sources of specimens include cancerous tissue from patients with primary hepatocellular carcinoma, tissues surrounding the cancer, and liver tissue obtained after skin puncture. (2) The extracted cell DNA is subjected to electrophoresis, and the entire high molecular weight DNA is transferred and immobilized on the DBM-cellulose filter paper after electrophoresis, dried and hybridized with the cloned HBV-DNA molecule labeled with 32P after purification. , only the DNA in the DNA that is strictly complementary to the HBV-DNA sequence is able to interact with ~(32)p HBV-