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目的针对衰老真皮成纤维细胞胶原基因表达减少的特点,应用胎儿皮肤源性活性蛋白(SCF)进行调控,以增加胶原基因的表达。方法应用真皮成纤维细胞培养、胶原基因mRNA裂隙杂交和图像分析技术,观察胎儿皮肤源性活性蛋白对真皮胶原基因的调控作用。结果以Actin作为对照,SCF对老人成纤维细胞胶原基因有明显的调控作用,可以使Ⅰ、Ⅲ型胶原基因表达增加,处理后,I型胶原基因表达增加150%,Ⅲ型胶原基因增加50%。结论SCF可以促进衰老真皮成纤维细胞胶原基因的表达。
Objective To study the characteristics of collagen gene expression in aging dermal fibroblasts and to control the expression of collagen genes by using the skin-derived active protein (SCF). Methods The dermal fibroblast culture, collagen mRNA fissure hybridization and image analysis techniques were used to observe the regulation of dermal collagen gene by fetal dermal active protein. Results With Actin as control, SCF could obviously regulate the collagen gene of human fibroblasts and increase the gene expression of type Ⅰ and type Ⅲ collagen. After treatment, the expression of type Ⅰ collagen gene increased by 150% and type Ⅲ collagen gene increased by 50% . Conclusion SCF can promote the collagen gene expression in aging dermal fibroblasts.