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目的:研究种植人参生长期施用三唑酮后,三唑酮在人参茎叶与根部残留的降解情况。方法:人参生长期施用正常浓度和高浓度的三唑酮农药,按一定的时间间隔取样,对人参茎叶和根样品分别进行前处理,以丙酮为提取溶剂,并采用凝胶渗透色谱(GPC)柱和活性炭固相萃取柱(ENVI-Carb-SPE)结合的方法进行样品净化,DB-5MS毛细管色谱柱程序升温分离,采用气质联用负化学电离源(NCI)选择离子检测(SIM)方式检测,以空白样品提取液配制对照品溶液,外标法测定。结果:将测定的数据进行回归处理,在茎叶中,喷施正常浓度三唑酮的回归方程为C=127686e-0.13t,r=0.91,T1/2=5.32d;喷施高浓度三唑酮的回归方程为C=17436e-0.11t,r=0.90,T1/2=6.15d。在根中,喷施正常浓度三唑酮的回归方程为C=243.39e-0.14t-203.37e-1.08t,Tmax=2.17d,Cmax=656.92μg.kg-1;喷施高浓度三唑酮的回归方程为C=79.43e-0.13t-313.93e-1.13t,Tmax=2.16d,Cmax=1053.55μg.kg-1。结论:三唑酮在人参茎叶中的动态曲线符合动力学一级降解模式C=e-kt,在人参根中的动态曲线符合一级吸收模式C=A(e-αt-e-βt)。
OBJECTIVE: To study the degradation of triadimefon residues in leaves and roots of ginseng after the application of triadimefon during the growth period of ginseng. Methods: Ginseng was treated with triadimefon at normal and high concentrations during the growth period of ginseng. Samples of ginseng stems, leaves and roots were pretreated at regular time intervals. Acetone was used as the extraction solvent and gel permeation chromatography (GPC ) Column and activated carbon solid-phase extraction column (ENVI-Carb-SPE) were combined to purify the sample. The temperature was separated by DB-5MS capillary column chromatography, and the SIM method Detection, the blank sample extract preparation reference solution, external standard method. Results: The regression equation of the measured data was that C = 127686e-0.13t, r = 0.91, T1 / 2 = 5.32d in spraying normal triadimefon in stems and leaves. The regression equation of ketone was C = 17436e-0.11t, r = 0.90 and T1 / 2 = 6.15d. In roots, the regression equation of spraying normal concentration of triadimefon was C = 243.39e-0.14t-203.37e-1.08t, Tmax = 2.17d, Cmax = 656.92μg.kg-1; spraying high concentration of triadimefon The regression equation was C = 79.43e-0.13t-313.93e-1.13t, Tmax = 2.16d, Cmax = 1053.55μg.kg-1. Conclusions: The dynamic curve of triadimefon in stem and leaf of ginseng accords with the kinetic first-order degradation model C = e-kt. The dynamic curve of root in ginseng root accords with the first-order absorption mode C = A (e-αt-e-βt) .