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试验选取苹果砧木难咽(M.micromalus Mak.)为试材,以快速繁殖为目的,对影响外植体建立、新梢增殖、生根及试管苗移栽的各个因素进行了研究。结果表明,外植体接种最适期为4-6月;最佳继代培养基为MS+BA0.5～1.0ppm+NAAO.05ppm+CH200ppm+蔗糖40g/1+琼脂6～8g/1;继代培养的最佳间隔时间是23～28d;取继代8次以上的试管苗嫩茎接种于1/2MS+IBA0.5ppm+蔗糖25g/1+琼脂6g/1的生根培养基上,生根率达90％以上;试管苗根系有根毛,移栽成活率85％,这对大量繁殖苗木具有应用价值。
In this study, M.micromalus Mak. Was chosen as the test material to study the factors influencing the establishment of explants, proliferation of shoots, rooting and transplanting of test tube seedlings for rapid propagation. The results showed that the optimal explant inoculation duration was from April to June. The best subculture medium was MS + BA 0.5 ~ 1.0ppm + NAAO 0.05ppm + CH 200ppm + sucrose 40g / 1 + agar 6 ~ 8g / 1. The optimal interval of on-line culture was 23-28 days. The young shoots of test-tube seedlings more than 8 generations were inoculated on rooting medium with 1 / 2MS + IBA 0.5ppm + sucrose 25g / 1 + agar 6g / More than 90%; root canal hairy root tissue culture, transplanting survival rate of 85%, which has a high value for breeding seedlings.