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目的探讨川芎嗪(TMP)对阿尔茨海默病(AD)模型小鼠脑胆碱乙酰基转移酶(ChAT)和N-甲基-D-天冬氨酸(NMDA)受体的影响。方法 56只小鼠随机分为五组:正常对照组(A组,10只)、D-半乳糖(D-gal)模型组(B组,10只)、TMP低、高剂量组(C、D组,各12只)和哈伯因阳性对照组(E组,12只)。Morris水迷宫测试学习记忆功能,同时进行大脑皮层、海马ChAT活性测定和NMDA受体结合试验。结果与A组相比,B组学习记忆能力和海马NMDA受体含量均下降(P<0.01);TMP可明显改善模型小鼠的学习记忆能力,增强皮层和海马ChAT活性,上调海马NMDA受体数目(P<0.05)。结论 TMP可明显提高模型小鼠的学习记忆功能,这可能与增强ChAT活性及保护NMDA受体有关。
Objective To investigate the effect of tetramethylpyrazine (TMP) on brain acetylcholinesterase (ChAT) and N-methyl-D-aspartate (NMDA) receptors in mice with Alzheimer’s disease (AD) Methods 56 mice were randomly divided into five groups: normal control group (group A, n = 10), D-galactose group (group B, n = 10), low TMP and high dose group D group, 12 rats each) and Habuine positive control group (E group, 12 rats). Morris water maze test learning and memory function, simultaneous cerebral cortex, hippocampal ChAT activity determination and NMDA receptor binding test. Results Compared with group A, the learning and memory abilities and NMDA receptor contents in hippocampus of group B were decreased (P <0.01). TMP significantly improved the learning and memory abilities of model mice, enhanced the activity of ChAT in cortex and hippocampus, increased the NMDA receptor Number (P <0.05). Conclusion TMP can significantly improve learning and memory in model mice, which may be related to enhancing ChAT activity and protecting NMDA receptor.