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目的 :调查我国不同地区、通过不同传播途径感染人类获得性免疫缺陷病毒I型 (HIV 1)患者中丙型肝炎病毒(HCV)的流行情况及不同亚型的分布。方法 :采用酶联免疫吸附试验 (ELISA)检测抗 HIV 1并以蛋白印迹试验 (Westernblot,WB)进行确认。采用DNA分支放大 (bDNA)技术检测HIV 1病毒载量 ,采用荧光抗体流式细胞检测技术 (FACs)作CD4和CD8细胞计数。抗 HCV检测采用ELISA方法。HCV基因亚型的测定采用实时 (real time)聚合酶链反应 (PCR)方法。结果 :共检测了 2 39例HIV 1感染者 ,抗 HCV阳性率为 5 6 .9%(136 / 2 39) ,其中经不同传播途径感染HCV的阳性率分别为 :静脉注毒 :42 .7%(5 8/ 136 ) ;经血液 :5 3.7%(73/ 136 ) ;性接触途径 :3.7%(5 / 136 )。静脉注毒者 (云南和新疆 )HCV感染以 1,3,4亚型最多 ,而输血人群 (河南省 )感染的HCV以 1,2亚型为主。结论 :HIV 1感染者中存在HCV混合感染 ,我国HCV基因亚型以 1型为主。
Objective: To investigate the epidemiology and distribution of hepatitis C virus (HCV) in different regions of China infected with human immunodeficiency virus type 1 (HIV 1) by different routes of transmission. Methods: Anti-HIV 1 was detected by enzyme-linked immunosorbent assay (ELISA) and confirmed by Western blot (WB). HIV 1 viral load was detected by DNA-branching amplification (bDNA), and CD4 and CD8 cell counts were measured by fluorescent antibody flow cytometry (FACs). Anti-HCV test using ELISA method. HCV genotypes were determined by real-time polymerase chain reaction (PCR). Results: A total of 239 HIV-1 patients were tested and the positive rate of anti-HCV was 56.9% (136/239). The positive rates of HCV infection by different routes of transmission were: intravenous injection of 42.7 % (58/135); blood: 53.7% (73/136); sexual route of exposure: 3.7% (5/136). HCV infection in Yunnan province and Xinjiang province was highest in subtype 1, 3 and 4, while HCV infection in Henan province and Henan province was mainly subtype 1 and 2. Conclusion: HCV co-infection exists in HIV-1 infected patients. HCV genotypes in China are mainly type 1.