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γ-氨基丁酸是哺乳动物中枢神经系统中的一种重要神经递质,通过活化受体连接的氯离子载体,并因此增加神经细胞膜对氯离子的通透性而产生抑制效应。本文报告了关于彻底冲洗原始培养的脑细胞膜中抑制性神经递质γ-[~3H)氨基丁酸与其受体位置结合的研究结果。必需彻底冲洗培养的脑细胞以除去γ-氨基丁酸受体结合的内源性抑制物。γ-[~3H]氨基丁酸对培养脑细胞的结合是特异性的,
GABA is an important neurotransmitter in the mammalian central nervous system and exerts an inhibitory effect by activating the receptor-linked chloride ionophore and thereby increasing the permeability of nerve cell membranes to chloride ions. This paper reports the results of studies on the combination of the inhibitory neurotransmitter gamma- [~ 3H) aminobutyric acid and its receptor in a thoroughly rinsed primate brain cell membrane. It is necessary to rinse the cultured brain cells thoroughly to remove endogenous inhibitors of γ-aminobutyric acid receptor binding. γ- [~ 3H] Aminobutyric acid is specific for the binding of cultured brain cells,