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用氩离子激光（波长５１４ｎｍ）照射，部花青（ＭＣ５４０）介导的光敏作用对白血病细胞系ＨＬ－６０、Ｋ５６２，急性髓细胞白血病原代祖细胞（ＣＦＵ－Ｌ）以及正常骨髓ＣＦＵ－ＧＭ的杀伤敏感性作了比较；用电镜观察处理前后Ｋ５６２细胞的超微结构变化。单纯激光照射或仅有ＭＣ５４０而不照光，对ＨＬ－６０细胞集落产率及ＣＦＵ－ＧＭ产率无明显影响。当ＭＣ５４０浓度为２５μｇ／ｍｌ、激光照射能量密度为７９．５１／ｃｍ￣２的条件处理，ＨＬ－６０集落产率下降２．８个对数级、ＣＦＵ－Ｌ减少２．２个对数级，而骨贿ＣＦＵ－ＧＭ尚能保留６４．０±７．０％；含１％Ｋ５６２细胞的模拟白血病骨贿经同样处理，Ｋ５６２集落产率能下降１．３６对数级，而ＣＦＵ－ＧＭ尚存７４．０±１９．０％。电镜观察发现处理后的Ｋ５６２细胞体积增大，肿胀，胞膜洞孔样破坏。结果提示，氩离子激光照射，ＭＣ５４０介导的光敏作用能选择性杀伤白血病细胞系，原代白血病祖细胞同样敏感。其对细胞的杀伤作用可能主要因直接造成胞膜破坏。该方法将可能成为一种有前途的自体骨髓移植物体外净化手段。
Irradiation with an argon ion laser (wavelength of 514 nm), merocyanine (MC540)-mediated photosensitization of the leukemia cell lines HL-60, K562, acute myeloid leukemia primary progenitor cells (CFU-L), and normal bone marrow CFU-GM The sensitivity of killing was compared; the ultrastructural changes of K562 cells before and after treatment were observed by electron microscopy. Laser irradiation alone or MC540 alone did not affect light, and there was no significant effect on the colony yield of HL-60 cells and the yield of CFU-GM. When the MC540 concentration was 25 μg/ml and the laser irradiation energy density was 79.51/cm 2 , the HL-60 colony yield decreased by 2.8 log and the CFU-L decreased by 2.2 logarithm. However, the bone bridging CFU-GM still retains 64.0±7.0%; the mimicking leukemia bone bridging with 1% K562 cells undergoes the same treatment, and the K562 colony yield can be reduced by 1.36 logarithm, while the CFU-GM The remaining 74.0±19.0%. Electron microscopy showed that the treated K562 cells increased in size, swelling, and membrane-like pore destruction. The results suggest that, with argon ion laser irradiation, MC540-mediated photosensitization can selectively kill leukemia cell lines, and primary leukemia progenitor cells are also sensitive. Its cytotoxic effect may be mainly due to direct membrane damage. This method may become a promising means of external purification of autologous bone marrow transplants.