[Objective] To establish the methods for the identification and determination of flavonoids components in Prinsepia utilis Royle.[Methods] Ethanol reflux extraction method was adopted to extract total flavonoids from Prinsepia utilis Royle,the physiochemical properties of which were identified by color reaction and thin-layer chromatography(TCL),the content of total flavonoids was determined by Al(NO3)-NaNO2 complexometry.[Results] The maximum absorption wavelength of sample solution was 510 nm and the solution was stable within 15 min.There was a good linear relationship between the concentration of rutin control solution(x) and absorbance value(y) with the linear regression equation of y=-2.571×10-3+13.163x,r=0.999 8;the average recovery rate was in the range of 98.00%-104.00%,101.16%was taken as average with RSD=2.15.It is detected that the flavonoids content in Prinsepia utilis Royle was 7.20%-7.30% with an average of 7.25%±0.05%,RSD=0.69%;the flavonoids content in oil meal of Prinsepia utilis Royle was 0.37%-0.38% with an average of 0.38%±0.01%,RSD=1.54%;The yield rate of flavonoids was 5.17%-5.25% with an average of 5.20%±0.04%,RSD=0.80%.[Conclusion] The method was simple,rapid,accurate and reliable,which could be applied to the determination of flavonoids in Prinsepia utilis Royle. [Objective] To establish the methods for the identification and determination of flavonoids components in Prinsepia utilis Royle. [Methods] Ethanol reflux extraction method was adopted to extract total flavonoids from Prinsepia utilis Royle, the physiochemical properties of which were identified by color reaction and thin -layer chromatography (TCL), the content of total flavonoids was determined by Al (NO3) -NaNO2 complexometry. [Results] The maximum absorption wavelength of sample solution was 510 nm and the solution was stable within 15 min. relationship between the concentration of rutin control solution (x) and absorbance value (y) with the linear regression equation of y = -2.571 × 10-3 + 13.163x, r = 0.999 8; the average recovery rate was in the range of 98.00 % -104.00%, 101.16% was taken as average with RSD = 2.15. It is detected that the flavonoids content in Prinsepia utilis Royle was 7.20% -7.30% with an average of 7.25% ± 0.05%, RSD = 0.69%; the flavonoids content in oil meal of Pr insepia utilis Royle was 0.37% -0.38% with an average of 0.38% ± 0.01%, RSD = 1.54%, and the yield rate of flavonoids was 5.17% -5.25% with an average of 5.20% ± 0.04%, RSD = 0.80%. [Conclusion] The method was simple, rapid, accurate and reliable, which could be applied to the determination of flavonoids in Prinsepia utilis Royle.