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目的 :通过基因重组建立高效表达有活性的ＰＡＬ菌株 ,为开创苯丙酮尿症的新疗法奠定基础。方法 :对已克隆于ｐＵＣ1 9质粒中的水稻苯丙氨酸氨解酶的ｃＤＮＡ进行反向测序 ,根据测序结果 ,与ｐＥＴ2 8系列质粒中的阅读框架结构进行比较 ,选择相匹配的ｐＥＴ2 8ｃ质粒作为表达载
OBJECTIVE: To establish an overexpressing PAL strain by gene recombination, which will lay the foundation for a new therapy for phenylketonuria. Methods: The cDNA of rice phenylalanine ammonia-lyase which has been cloned in pUC19 plasmid was reverse-sequenced and compared with the reading frame structure of pET28 plasmid according to the sequencing results. The matched pET2 8c plasmid As an expression load