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目的探讨Raf激酶抑制蛋白(RKIP)在肺癌组织中的表达及其与肺癌侵袭转移的相关性。方法采用RT-PCR和Western blot方法检测95例肺癌患者的肺癌组织及其癌旁正常肺组织中RKIP的表达,分析其与肺癌临床病理学特征的关系。结果肺癌组织RKIP mRNA阳性表达率和蛋白表达量均低于正常肺组织(48.42%vs.77.89%和0.598±0.124vs.0.623±0.116),肺癌中、低分化组均低于高分化组[(39.47%、20.00%vs.66.67%)和0.318±0.117、0.104±0.005vs.0.623±0.116),伴有淋巴结转移组低于无淋巴结转移组(31.71%vs.61.11%和0.309±0.112vs.0.737±0.175),生存期<2年者低于生存期≥2年者(34.78%vs.61.22%和0.302±0.107vs.0.742±0.225)(P<0.05)。结论 RKIP的低表达可能与肺癌的发生及侵袭转移有关。
Objective To investigate the expression of Raf kinase inhibitor (RKIP) in lung cancer and its correlation with the invasion and metastasis of lung cancer. Methods RT-PCR and Western blot were used to detect the expression of RKIP in 95 lung cancer patients and their adjacent normal lung tissues. The relationship between them and clinicopathological features of lung cancer was analyzed. Results The positive rates of RKIP mRNA and protein expression in lung cancer tissues were lower than those in normal lung tissues (48.42% vs 77.89% and 0.598 ± 0.124vs.0.623 ± 0.116, respectively) 39.47%, 20.00% vs.66.67%) and 0.318 ± 0.117,0.104 ± 0.005 vs.0.623 ± 0.116 respectively), with lymph node metastasis was lower than that without lymph node metastasis (31.71% vs.61.11% and 0.309 ± 0.112 vs.0.737 ± 0.175), those with survival less than 2 years were lower than those with survival ≥ 2 years (34.78% vs.61.22% and 0.302 ± 0.107 vs.0.742 ± 0.225 respectively) (P <0.05). Conclusion The low expression of RKIP may be related to the occurrence and invasion of lung cancer.