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为了阐明PGE_1的作用机制并对新的PGE_1类似物——一个口服有效的事后避孕药CP_(48630)的作用进行评价,作者做了以下实验。选择12只妊娠43天的豚鼠,6只为实验组,上午11时口服1mg的CP_(48630)(溶于1ml溶媒中),另6只给予溶媒(85mg Myrj53+100mgNacl+100mlH_2O)作为对照。如5小时内不流产下午4时再给1mgCP_(48630)。2个实验豚鼠和2个对照豚鼠在给CP_(48630)前后用微囊技术(AI Csapa,1970)监护子宫内压力(IUP),持续观察以上12只豚鼠直到实验组鼠分娩第一只仔鼠,此时将实验豚鼠麻醉,开腹并收集以下标本:子宫静脉血、心脏血、卵巢、子宫和胎盘。将4ml血样品低温离心,得2ml
In order to elucidate the mechanism of action of PGE_1 and evaluate the role of the new PGE_1 analogue, CP_ (48630), an orally available and effective contraceptive, the authors performed the following experiment. Twelve guinea pigs were selected on the 43th day of gestation. Six of them were in the experimental group. At 11am, CP 1 (48630) was orally administered in 1ml of vehicle and the other 6 were given vehicle (85mg of Myrj53 + 100mg of NaCl + 100ml of H 2 O). If no abortion within 5 hours at 4 pm to give 1mgCP_ (48,630). Two experimental guinea pigs and two control guinea pigs monitored intrauterine pressure (IUP) with microcapsule technique (AI Csapa, 1970) before and after administration of CP 48630 and continued to observe the above 12 guinea pigs until the first pup gave birth The guinea pigs were anesthetized and anastomosed and the following specimens were collected: uterine venous blood, cardiomyocytes, ovaries, uterus and placenta. 4ml blood samples were centrifuged at low temperature to obtain 2ml