目的分离和测定联邦止咳露中 3种药物的含量。方法用高效液相色谱法测定联邦止咳露中磷酸可待因、盐酸麻黄碱、扑尔敏的含量。柱规格 :Kmmasil柱 (5 μm ,15 0mm× 4.6mm ,i.d .) ,流动相组成甲醇∶水∶四氢呋喃∶ 85 %磷酸 (36 0∶ 6 48∶ 5 0∶ 2 )。LC 480二极管阵列检测器。结果磷酸可待因 ,盐酸麻黄碱和扑尔敏的回收率和相对标准偏差分别为 98.85 % ,0 .98% ;97.30 % ,1.10 %和 98.2 1% ,0 .92 % (n =5 )。结论用本法分离和测定以上 3种药物是行之有效的 Objective To isolate and determine the contents of three drugs in the Cough Syrup. Methods The contents of codeine phosphate, ephedrine hydrochloride and chlorpheniramine in Cough Syrup were determined by high performance liquid chromatography. Column specifications: Kmmasil column (5 μm, 150 mm × 4.6 mm, i.d.), mobile phase consisting of methanol: water: tetrahydrofuran: 85% phosphoric acid (36 0: 6 48: 5 0: 2). LC 480 Diode Array Detector. Results The recoveries and relative standard deviations of codeine phosphate, ephedrine hydrochloride and chlorphenamine were 98.85%, 0.98%, 97.30%, 1.10% and 98.2 1%, 0.92% (n = 5), respectively. Conclusion The method of separation and determination of the above three drugs is effective