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目的优化酶解-吸附澄清法提取水辣蓼总黄酮的工艺。方法采用响应面法,构建多元二次回归数学模型,筛选酶法提取和絮凝澄清的最佳工艺条件。结果最佳酶法提取工艺为料液比1∶50,p H6.06,复合酶用量0.24%,酶解温度50℃,酶解时间1.62 h;最佳澄清工艺为酶解液的浓缩比1∶5,药液p H5.24,膨润土用量0.45 m L·g-1,43.43℃下絮凝40 min。结论酶法提取条件温和,膨润土絮凝能有效降低酶解液中总固形物的含量,优于传统醇沉法。
OBJECTIVE To optimize the technology of extracting total flavonoids from Polygonum capitatum by enzymolysis - adsorption clarification. Methods The response surface method was used to construct the mathematical model of multivariate quadratic regression, and the optimum technological conditions of enzymatic extraction and flocculation clarification were screened. Results The optimal enzymatic extraction process was as follows: solid-liquid ratio 1:50, p H6.06, enzyme dosage 0.24%, enzymolysis temperature 50 ℃, hydrolysis time 1.62 h; : 5, liquid p H5.24, the amount of bentonite 0.45 m L · g-1, 43.43 ℃ under flocculation 40 min. Conclusions Enzymatic extraction conditions are mild, and bentonite flocculation can effectively reduce the total solid content in the hydrolyzate, which is better than the traditional alcohol precipitation method.