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目的探讨胰岛因子1(ISL1)在维吾尔族妇女宫颈病变组织中的表达情况及其临床意义。方法采用半定量RT-PCR技术检测ISL1在维吾尔族妇女宫颈鳞癌(CSCC,15例)、宫颈上皮内瘤变(CINⅡ～Ⅲ,13例)和正常宫颈组织(12例)中的mRNA表达情况;采用免疫组织化学(S-P)法检测维吾尔族妇女CSCC(42例)、CINⅢ(23例)和正常宫颈组织(20例)中ISL1蛋白的表达情况。结果 CSCC组织中ISL1的mRNA表达水平(0.153 0±0.122 3)明显低于正常宫颈组织(0.271 3±0.763 3),差异有统计学意义(P<0.05),CINⅡ～Ⅲ组织中ISL1的mRNA转录表达水平(0.225 3±0.132 5)与CSCC及正常宫颈组织比较,差异均无统计学意义(P>0.05)。免疫组织化学分析结果显示,CSCC组织中的ISL1蛋白表达水平(16.67%)显著低于正常宫颈组织(55.00%),差异有统计学意义(P<0.01),CINⅢ组织中ISL1的表达水平(26.09%)与CSCC及正常宫颈组织比较,差异均无统计学意义(P>0.05)。结论 ISL1蛋白和mRNA在维吾尔族妇女宫颈癌中呈低表达,对该基因进行检测有利于宫颈癌的早期诊断、早期筛查。
Objective To investigate the expression of islet 1 (ISL1) in cervical lesions of Uygur women and its clinical significance. Methods Semi-quantitative RT-PCR was used to detect the mRNA expression of ISL1 in Uigur women with cervical squamous cell carcinoma (CSCC, 15 cases), cervical intraepithelial neoplasia (CINⅡIII, 13 cases) and normal cervical tissue (12 cases) Immunohistochemistry (SP) was used to detect the expression of ISL1 protein in Uighur women with CSCC (42 cases), CINⅢ (23 cases) and normal cervical tissues (20 cases). Results The mRNA expression level of ISL1 in CSCC tissues (0.153 0 ± 0.122 3) was significantly lower than that in normal cervical tissues (0.271 3 ± 0.763 3) (P <0.05). ISL1 mRNA transcription in CIN Ⅱ ~ Ⅲ tissues The expression level (0.225 3 ± 0.132 5) was no significant difference between CSCC and normal cervical tissue (P> 0.05). The results of immunohistochemistry showed that the expression level of ISL1 protein in CSCC tissues (16.67%) was significantly lower than that in normal cervical tissues (55.00%), the difference was statistically significant (P <0.01) %) Compared with CSCC and normal cervical tissue, the difference was not statistically significant (P> 0.05). Conclusion ISL1 protein and mRNA are low expressed in Uighur women with cervical cancer. Detection of this gene is helpful for the early diagnosis and early screening of cervical cancer.