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“灰心病”是近几年常见的大白菜严重病害,其病症主要特点是包心初期病菜内部叶片显现灰褐色坏死斑点。我们曾报导过,由“灰心菜”症状的大白菜病株中,分离出一种属于烟草花叶病毒株系的大白菜病毒。为了进一步研究这种病毒与“灰心病”的关系,现将病毒纯化制剂进行回接试验。用适量pH7.2、0.2M磷酸缓冲液稀释大白菜病毒纯化制剂,按常规汁液接种法接种于天津绿、青什中锋、城阳青等大白菜上,其发病率和病情指数见下表。
The “discouraged disease” is a serious disease of common Chinese cabbage in recent years. The main feature of the disease is the appearance of beige necrotic spots on the internal leaves of diseased vegetables. We have reported that a Chinese cabbage virus belonging to the tobacco mosaic virus strain was isolated from Chinese cabbage strains that are “discouraged”. To further investigate the relationship of this virus to “heart disease,” the virus-purified preparations are now back-patched. Chinese cabbage virus was diluted with suitable pH7.2,0.2M phosphate buffer and inoculated to Chinese cabbage, such as Tianjin Green, Qingshi Center and Chengyang Qing, according to the conventional method of inoculation. The incidence and disease index are shown in the following table.