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目的运用ATP生物荧光法验证骨科植入物不同清洗流程的有效性及效果。方法随机选取不同厂家的骨科植入物包括骨钉、接骨板共64件,分为A组30件、B组34件,先运用ATP生物荧光法检测初始ATP值,确认初始污染程度相对平衡,然后使用两种清洗流程分别清洗A组、B组植入物,完成后再运用ATP生物荧光法检测清洗后的ATP值,比较A、B两种清洗方法的有效性。结果 A、B两组植入物初始污染物无统计学意义(P>0.05),污染程度相对一致。清洗后的植入物B组ATP值检测合格率为97.1%,A组合格率仅为66.7%。B组清洗合格率明显高于A组(P<0.01)。结论通过本实验,验证B组方法简便易行,依从性高,是针对骨科植入物更有效的清洗流程,能取得清洗的更高质量,保障植入物灭菌的最终效果。而ATP生物荧光法检测手段在骨科植入物清洗效果监测中,可操作性强、简便、快捷,是一种目前在消毒供应中心对清洗质量监测的有效方法。
Objective To validate the effectiveness of different cleaning procedures of orthopedic implants by ATP biofluorescence. Methods A total of 64 orthopedic implants including bone nail and bone plate from different manufacturers were randomly divided into group A (30 in group A) and group B (group B). The initial ATP value was determined by ATP biofluorescence method to confirm the relative degree of initial pollution. Then, two kinds of cleaning procedures were used to clean the implants of Group A and Group B, respectively. After completion, the ATP value after cleaning was detected by ATP biofluorescence. The effectiveness of two cleaning methods A and B was compared. Results The initial implants of implants in groups A and B had no statistical significance (P> 0.05), and the degree of contamination was relatively consistent. The pass rate of ATP value in group B after cleaning was 97.1%, and the passing rate of group A was only 66.7%. B group cleaning pass rate was significantly higher than the A group (P <0.01). Conclusion Through this experiment, it is proved that the method of group B is simple and easy to operate and has high compliance. It is a more effective cleaning procedure for orthopedic implants, and can achieve higher quality of cleaning and ensure the final effect of sterilizing implants. However, ATP biofluorescence assay is an effective method for monitoring the quality of cleaning in orthopedic implant cleaning because of its maneuverability, convenience and speed.