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目的 观察黄芪总多糖在活体中的抗人疱疹病毒I型的作用。方法 以阿昔洛韦 (ACV)为阳性对照 ,以磷酸缓冲的盐水 (phosphatebufferedsaline ,PBS)以及含 10 %DMSO (二甲亚砜 )的PBS为阴性对照 ,用HSV - 1HS - 1株感染豚鼠皮肤的动物模型 ,观察了黄芪总多糖抗HSV - 1药效。结果 ACV、黄芪总多糖处理皮区的累积计分分别为 14 2 0± 6 70、 12 75± 3 5 8。ACV、黄芪总多糖处理皮区的痊愈天数分别为 7 70± 1 0 8、 8 5 0± 0 5 0 ;与PBS及含 10 %DMSO的PBS处理皮区之差异 ,有统计学意义 (P <0 0 5 )。HSV - 1HS - 1株感染过程中 ,各处理皮区的大体照片及皮肤样本的病毒滴度检测 ,获得了一致的结果。ACV与黄芪总多糖处理皮区的累积计分、痊愈天数 ,以及两种方法处理皮区皮肤样本的病毒检测结果 ,其差异均无统计学意义 (P >0 0 5 )。结论 黄芪总多糖与ACV药效相近 ,值得开发利用。
Objective To observe the effect of total polysaccharide from Astragalus membranaceus on human herpesvirus I in vivo. Methods Acyclovir (ACV) as a positive control, phosphate buffered saline (PBS) and PBS containing 10% DMSO (dimethylsulfoxide) as negative controls, and HSV-1HS-1 strain infecting guinea pig skin The animal model was observed for total anti-HSV-1 efficacy of Astragalus polysaccharides. Results The accumulative scores of ACV and Astragalus polysaccharide treated areas were 14 2 0 ± 6 70 and 12 75 ± 3 5 8, respectively. The number of recovery days of ACV and Astragalus polysaccharide treated dermatomedial areas were 7 70±108, 850±0.50, respectively; the difference between PBS and PBS treated with PBS and 10% DMSO was statistically significant (P < 0 0 5 ). During the infection of HSV-1HS-1 strains, the general photo of the treated areas and the virus titre of the skin samples were tested and consistent results were obtained. There were no statistically significant differences (P > 0 0 5) between the accumulative scoring of the skin area treated with ACV and Astragalus polysaccharides, the days of recovery, and the virus detection results of the skin samples from the two methods. Conclusion Astragalus polysaccharides and ACV are similar in efficacy and worthy of development and utilization.