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目的研究帕金森病（PD）黑质多巴胺神经元的死亡机制。方法用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺（DA）和还原型谷胱甘肽（GSH）对PC12细胞凋亡的影响。结果发现DA可诱发PC12细胞凋亡，适中浓度时（0．45mmol／L）PC12细胞凋亡数最多（凋亡率为48．7％±6．3％）；抗氧化剂还原型合胱甘肽（GSH）可部分阻止DA诱发的PC12细胞凋亡（P＜0．01）。结论凋亡参与了PD的病变过程，采用适当的抗氧化剂对于PD治疗具有积极的意义。
Aim To study the mechanism of death of dopaminergic neurons in Parkinson’s disease (PD). Methods The effects of dopamine (DA) and reduced glutathione (GSH) on the apoptosis of PC12 cells were observed by fluorescence microscopy with DNA labeling. The results showed that DA could induce the apoptosis of PC12 cells, and the apoptosis rate of PC12 cells was the highest at the moderate concentration (0.45mmol / L) (the apoptosis rate was 48.7% ± 6.3%). Antioxidant reduced glutathione (GSH) could partially prevent the apoptosis of PC12 cells induced by DA (P <0.01). Conclusion Apoptosis is involved in the pathological changes of PD. It is of positive significance to use PD as an appropriate anti-oxidant.