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目的 :观察一氧化氮合酶阳性神经元在正常大鼠脊髓中间带的分布。方法 :用正常成年 Wistar大鼠脊髓 ,采用冰冻切片 ,以尼可酰胺腺嘌呤二核苷酸磷酸脱氢酶 (NADPH- d)免疫组织化学方法 ,观察一氧化氮合酶阳性神经元在脊髓中间带的分布。结果 :在正常 Wistar大鼠中 ,一氧化氮合酶阳性神经元主要分布在脊髓的中间外侧核本部区、中介核和中央管周围。结论 :了解一氧化氮合酶阳性神经元在中间带的分布 ,可能对进一步了解自发性高血压等病理情况下的变化有参考价值。
Objective: To observe the distribution of nitric oxide synthase positive neurons in the spinal cord of normal rats. Methods: The normal adult Wistar rats spinal cord were harvested and frozen sections were used to observe the expression of nitric oxide synthase (NOS) -positive neurons in the middle of spinal cord by NADPH-d immunohistochemistry. Belt distribution. Results: In normal Wistar rats, nitric oxide synthase positive neurons mainly distributed in the medial lateral basal ganglia, medulla oblongata and central canal of the spinal cord. CONCLUSION: Understanding the distribution of nitric oxide synthase (NOS) positive neurons in the middle zone may be of reference value for further understanding of the pathological changes such as spontaneous hypertension.