免疫脂质体(immunoliposome)系脂质体与特异性抗体连接而成,具有普通脂质体和导向药物二者的优点。其中的药物活性保持好,携药量大,并能与靶细胞多价结合,因而在肿瘤导向治疗领域日益受到重视。 作者采用本室制备的胃癌单克隆抗体MGb_2经NHSP修饰引入软脂酸,逆相蒸发法制备脂质体,同时包入阿霉素(adriamycin)。用去氧胆酸透析法将修饰抗体插到阿霉素脂质体表面,经过Scpharose 4B柱层析纯化得到免疫脂质体。同 Immunoliposomal liposomes are linked to specific antibodies and have the advantages of both common liposomes and targeted drugs. Among them, the drug activity is kept well, the drug load is large, and it can be multi-valently combined with target cells, and thus it is increasingly valued in the field of tumor-directed therapy. The authors used the gastric cancer cell monoclonal antibody MGb2 prepared in this laboratory to be introduced into palmitic acid by NHSP modification, and the liposomes were prepared by reversed phase evaporation method. At the same time, adriamycin was included. The modified antibody was inserted into the surface of doxorubicin liposomes by diafiltration with deoxycholic acid and purified by Scpharose 4B column chromatography to obtain immunoliposomes. with