提出邻联茴香胺-H_2O_2-HRP伏安酶联免疫分析新体系,并用于测定HRP和HRP标记物.该方法是将HRP催化H_2O_2氧化邻联茴香胺的酶催化反应与邻联茴香胺的氧化产物的电极还原反应相偶合,在BR缓冲溶液中,在-0.56V(SCE)左右产生灵敏的极谱波.应用此极谱波测定HRP的检测限为3.7×10~(-12)g/mL,线性范围为1.O×1O~(-11)～2.0×10~(-9)g/mL.对邻联茴香胺-H_2O_2-HRP伏安酶联免疫分析新体系的偶合反应机理及电极还原过程进行了较详细的探讨. The new system of voltammetric enzyme immunoassay for the determination of HRP and HRP was proposed. The method was characterized by HRP catalyzed oxidation of o-dianisidine with H 2 O 2 and the oxidation of o-anisidine The results showed that the detection limit of HRP was 3.7 × 10 ~ (-12) g / L when the polarographic wave was used to generate sensitive polarographic wave at -0.56V (SCE) in BR buffer solution, mL and the linear range was 1.O × 10 ~ (-2) ~ 2.0 × 10 ~ (-9) g / mL. The coupling reaction mechanism of the new system for the enzyme linked immunosorbent assay (ELISA) with o-dianisidine-H_2O_2-HRP Electrode reduction process conducted a more detailed discussion.