论文部分内容阅读
以野生松潘乌头块根为材料,进行愈伤组织诱导与植株再生培养。由于松潘乌头离体培养时,组织褐变严重,因此在愈伤组织诱导前须进行除褐培养,培养基以MS+6-BA 1.0 mg·L-1+VC 300 mg·L-1+PVP 200 mg·L-1效果较好,连续转移3次后褐变率显著降低到10%。适宜的愈伤组织诱导培养基为MS+2,4-D 3.0 mg·L-1+6-BA 2.0 mg·L-1+LH 500 mg·L-1,诱导率100%;不定芽分化培养基为MS+ZT 1.0 mg·L-1+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1,分化率93%;不定芽增殖培养基为MS+6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1,平均增殖倍数为6.0左右;生根培养基为1/2MS+IAA 0.3 mg·L-1+VC 100 mg·L-1(或PVP 300 mg·L-1),平均生根数10条左右,生根率为96%以上。将瓶苗移栽于森林土和蛭石以1:1(V/V)混合的基质中,生长良好,成活率达90%以上。
The wild pine panoply root tuber was used as the material to induce callus induction and plant regeneration culture. Because of the serious tissue browning, the explants should be cultured in vitro with MS + 6-BA 1.0 mg · L -1 + VC 300 mg · L -1 + PVP 200 mg · L-1 is better, and the browning rate is significantly reduced to 10% after 3 successive transfers. The suitable medium for callus induction was MS + 2,4-D 3.0 mg · L-1 + 6-BA 2.0 mg · L -1 + LH 500 mg · L -1 with 100% induction rate. Differentiation of adventitious buds The basal medium was MS + ZT 1.0 mg · L-1 + 6-BA 2.0 mg · L-1 + NAA 0.5 mg · L-1 with a differentiation rate of 93%. MS + 6-BA 2.0 mg · L -1 + NAA 0.3 mg · L-1, and the average proliferation rate was about 6.0. The rooting medium was 1/2 MS + IAA 0.3 mg · L -1 + VC 100 mg · L -1 or PVP 300 mg · L -1 ), The average number of rooting about 10, rooting rate of 96% or more. The seedlings were transplanted in the matrix mixed with forest soil and vermiculite in the ratio of 1: 1 (V / V), which grew well and the survival rate was over 90%.