论文部分内容阅读
以聚ε-己内酯二元醇(PCL)为软段,赖氨酸二异氰酸酯(LDI)和胱胺(cystamine)为硬段,合成了以胱胺封端的线性聚氨酯预聚体,然后利用α-甲氧基-ω-环氧丙基聚乙二醇(EO-MPEG)的环氧基与胱胺的胺基反应,制备了含有二硫键的双亲性聚氨酯.利用GPC测试了聚氨酯的分子量,1H-NMR及FTIR表征了聚氨酯的结构.利用透析法制备了聚氨酯胶束,荧光探针技术测得其临界胶束浓度为5.6×10-3g/L,TEM分析表明胶束形貌为球形,DLS分析表明胶束的平均粒径为82 nm.还原响应实验表明胶束在10 mmol/L的谷胱甘肽的作用下粒径明显增大,表明胶束具有还原刺激响应特性.最后,以抗肿瘤疏水药物氨甲喋呤(MTX)为模型药物,研究了载药胶束在谷胱甘肽作用下的药物释放,结果表明谷胱甘肽的存在可刺激药物的释放,该材料可作为细胞内还原响应的载体材料使用.
A linear polyurethane prepolymer with cystamine capped was synthesized by using poly-ε-caprolactone diol (PCL) as soft segment, lysine diisocyanate (LDI) and cystamine as hard segment, The epoxy groups of α-methoxy-ω-epoxypropyl polyethylene glycol (EO-MPEG) were reacted with amine groups of cystamine to prepare disulfide-containing amphiphilic polyurethanes. The urethane The structure of polyurethane was characterized by 1H-NMR and FTIR. The polyurethane micelles were prepared by dialysis method. The critical micelle concentration was 5.6 × 10-3g / L measured by fluorescence probe technique. The TEM analysis showed that the morphology of micelles was Spherical, DLS analysis showed that the average size of the micelles was 82 nm. Reduction response experiments showed that micelles in the 10 mmol / L of glutathione significantly increased particle size, indicating that micelles have a reduction stimulus response characteristics Finally , The anti-tumor hydrophobic drug methotrexate (MTX) was used as a model drug to study the drug release of drug-loaded micelles under the action of glutathione. The results showed that the presence of glutathione stimulated the release of drugs, which can be used as a cell The internal reduction response carrier material is used.