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目的研究慢性乙型肝炎(CHB)患者HBV-DNA复制水平、肝功能生化指标及其与肝组织病理学相关性。方法采用荧光定量PCR法监测200例慢性乙型肝炎患者血清HBV-DNA含量及相关血清肝功能指标,并对患者活体肝穿刺的病理标本进行组织炎症活动度分级(G),结果利用统计软件统计分析。结果慢性乙型肝炎轻、中、重度组患者HBV-DNA含量分别为(4.85±1.0)×108~107copy/ml、(3.82±0.8)×106~105copy/ml、(1.8±0.5)×105~104copy/ml;血清总胆红素含量分别为(12.64±4.25)μmol/L、(50.18±9.46)μmol/L、(262.96±30.16)μmol/L;白/球比值分别是(1.51±0.23)、(1.61±0.26)和(1.97±0.25)。血清TB、ALT、AST、GGT平均值随病理分级和分期的增加而逐渐升高;ALB、PTA则相反。结论慢性乙型肝炎轻、中、重度各组之间的HBV-DNA含量差异有统计学意义;中、重度组病毒含量与总胆红素水平呈负相关,与白/球比值呈正相关。血清TB、ALT、AST、GGT可反映慢性乙型肝炎肝组织炎症活动的程度;ALB、PTA是慢性乙型肝炎病变进展的指标。
Objective To study the level of HBV-DNA replication, the biochemical markers of liver function and its relationship with liver histopathology in patients with chronic hepatitis B (CHB). Methods The levels of serum HBV-DNA and serum-related liver function in 200 patients with chronic hepatitis B were detected by real-time PCR. The histopathological grading (G) was performed on the biopsy specimens of patients with liver biopsy. The results were statistically analyzed using statistical software analysis. Results The HBV-DNA levels in patients with mild, moderate and severe chronic hepatitis B were (4.85 ± 1.0) × 108 ~ 107copy / ml, (3.82 ± 0.8) × 106 ~ 105copy / ml and (1.8 ± 0.5) × 105 ~ The levels of total bilirubin in serum were (12.64 ± 4.25) μmol / L and (50.18 ± 9.46) μmol / L and (262.96 ± 30.16) μmol / L, , (1.61 ± 0.26) and (1.97 ± 0.25), respectively. Serum TB, ALT, AST, GGT average with the pathological grading and staging increased gradually; ALB, PTA is the opposite. Conclusions The HBV-DNA content of the mild, moderate and severe groups of chronic hepatitis B has statistical significance. The virus content of moderate and severe groups is negatively correlated with the level of total bilirubin and has a positive correlation with the white / Serum TB, ALT, AST, GGT can reflect the degree of chronic hepatitis B liver inflammation; ALB, PTA is an indicator of the progress of chronic hepatitis B.