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目的通过显微外科技术建立小鼠原位肺移植模型,为肺移植研究提供动物模型。方法采用C57BL/6小鼠作为供、受体,行同基因小鼠原位左肺移植,使用Cuff套管法进行气管及血管吻合。术后7、14、21、28 d取移植肺及原肺,行HE染色,评价肺移植后效果。结果学习曲线后,共30例小鼠移植,手术成功率89%,小鼠成活率100%。供体手术时间:(35.2±9.81)min,受体手术时间:(24.6±7.42)min,冷缺血时间是:(46.6±8.92)min,热缺血时间是:(17.2±3.08)min。同基因移植物大体及病理无明显改变,病理显示与原肺无差别。结论本技术能够方便快捷建立小鼠肺移植模型,成功率高,可重复性强,符合原位肺移植临床生理,是研究肺移植发病机制和治疗的良好动物模型。
Objective To establish a mouse model of orthotopic lung transplantation by microsurgery and provide an animal model for lung transplantation. Methods C57BL / 6 mice were used as donor and recipient for the in situ left lung transplantation in allogeneic mice. Tracheal and vascular anastomoses were performed using Cuff cannula. At 7, 14, 21 and 28 days after operation, the grafted lung and primary lung were harvested for HE staining to evaluate the effect of lung transplantation. Results After the learning curve, a total of 30 mice were transplanted. The success rate of surgery was 89% and the survival rate of mice was 100%. The donor’s operation time was (35.2 ± 9.81) min, the operation time of the recipient was (24.6 ± 7.42) min, the cold ischemia time was (46.6 ± 8.92) min and the warm ischemia time was (17.2 ± 3.08) min. The same with the general and pathological grafts no significant change in pathology showed no difference with the original lung. Conclusion This technique can be used to establish mouse lung transplantation model conveniently and quickly with high success rate and strong repeatability. It is in line with the clinical physiology of lung transplantation in situ and is a good animal model to study the pathogenesis and treatment of lung transplantation.