Caulfield曾推论棉酚是蛋白激酶C的一种抑制剂.但迄今尚未见到直接论证这一设想的报告.作者将大鼠肝脏匀浆,经硫酸铵盐析、DEAE纤维素柱层析等,得到部分纯化的蛋白激酶C.将此酶制剂在体外与棉酚孵育,通过测定~(32)P由γ-~(32)P-ATP参入组蛋白的数量来计算蛋白激酶C的活性.结果表明,(-)棉酚以剂量依赖方式抑制蛋白激酶C活性,当其浓度为40、20、10、5和1μmol/L时,蛋白激酶C活性分 Caulfield has argued that gossypol is an inhibitor of protein kinase C. However, no report has been published so far on the hypothesis that the rat liver homogenate, ammonium sulfate salting-out, DEAE cellulose column chromatography, To obtain partially purified protein kinase C. The enzyme preparation was incubated with gossypol in vitro and the activity of protein kinase C was calculated by measuring the amount of? -32 P-ATP incorporated into histone by ~ (32) P. It was shown that (-) gossypol inhibited protein kinase C activity in a dose-dependent manner, and at concentrations of 40, 20, 10, 5 and 1 mol / L protein kinase C activity