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采用VP-ODS C18不锈钢柱,以乙腈-水(体积比15∶85)为流动相,流速0.6mL/min,光电二极管阵列检测器,检测波长299nm,柱温40℃,建立了吡蚜酮在水稻中的残留分析方法。样品经乙腈-氨水(体积比7∶3)混合溶液提取、二氯甲烷萃取,用装有无水硫酸钠、弗罗里硅土和活性炭的柱层析净化后,进行高效液相色谱检测。该方法的最小检出量为1.0×10-10g,最低检出质量分数为0.02mg/kg,稻杆、谷壳和糙米的平均添加回收率分别为83.62%～90.56%、88.00%～92.33%和89.40%～92.07%。
A VP-ODS C18 stainless steel column was used to establish the pymetrozine in the presence of acetonitrile-water (volume ratio 15:85) with a flow rate of 0.6 mL / min and a photodiode array detector at a detection wavelength of 299 nm at a column temperature of 40 ℃ Residue analysis in rice. The sample was extracted with a mixture of acetonitrile and ammonia (7: 3 by volume), extracted with dichloromethane and purified by column chromatography on anhydrous sodium sulfate, Florisil and activated charcoal before being analyzed by high performance liquid chromatography. The minimum detectable amount of this method was 1.0 × 10-10g, the minimum detectable mass fraction was 0.02mg / kg, the average recoveries of rice straw, chaff and brown rice were 83.62% -90.56%, 88.00% -92.33% And 89.40% ~ 92.07%.