前言目前柑桔病毒病的严重性已引起国内外的普遍重视,正多方面探索无毒化苗木的生产。其中一种方法是基于 White 等(1934)研究的结果,这就是病毒在植物体内的不均匀分布,顶端分生组织几乎不含病毒。Murashige(1972)首先在柑桔类植物中应用显微茎尖殊接技术,它不仅成功地排除了病毒,获得柑桔无病毒苗,而且还避免了来自于直接分离茎尖的多 Preface The severity of citrus virus disease has aroused widespread attention both at home and abroad and is exploring the production of non-toxic seedlings in many aspects. One of these approaches is based on the results of White et al.’s (1934) study. This is an inhomogeneous distribution of the virus in the plant, with apical meristems almost free of viruses. Murashige (1972) first used a microscopic shoot tip splicing technique in citrus plants, which not only successfully ruled out the virus but also obtained citrus-free virus seedlings but also avoided the need to separate directly from the shoot tips