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目的：研究鼻咽癌中１６号染色体的缺失情况。方法：采用显微切割的方法获取肿瘤组织，然后用ＰＣＲ的方法以 １６号染色体上的 ８对引物对 ３８例鼻咽癌进行分析。结果： ３８例鼻咽癌组织中所有标本至少有一个位点出现有杂合性缺失，其中 Ｄ１６５５３３出现杂合性缺失的频率最高，占 ８６．１％（３１／３３），此外，杂合性缺失频率超过５０％的有引物 Ｄ１６Ｓ３９８、Ｄ１６Ｓ３９０和 Ｄ１６Ｓ４２０，分别占 ７８.８％（２６／３３）、６９.４％（２５／３３）和 ５７． ６％（１９／３３）。结论：本研究首次发现在 Ｉ６号染色体上存在三个杂合性缺失高频缺失区，分别位于 １６ｐ１２．３（引物 Ｄ１６Ｓ４２０）。１６ｑ１２.２（引物Ｄ１６Ｓ３９０）和 １６ｑ２１－２２．１（引物Ｄ１６Ｓ５３３，Ｄ１６Ｓ３９８）。
Objective: To study the deletion of chromosome 16 in nasopharyngeal carcinoma. Methods: Tumor tissues were obtained by microdissection, and then 38 nasopharyngeal carcinomas were analyzed by PCR with 8 pairs of primers on chromosome 16. Results: There was heterozygous deletion in at least one locus in all 38 specimens of nasopharyngeal carcinoma. The frequency of heterozygosity deletion in D165533 was the highest (86.1%, 31/33). In addition, heterozygosity The primers D16S398, D16S390 and D16S420 with deletion frequencies of over 50% accounted for 78.8% (26/33) and 69.4% (25/33), respectively. 6% (19/33). CONCLUSION: This study is the first to find three heterozygous deletion high frequency deletion regions on chromosome I6, located at 16p12.3 (primer D16S420). 16q12.2 (primers D16S390) and 16q21-22.1 (primers D16S533, D16S398).