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目的:研究鼻咽癌中16号染色体的缺失情况。方法:采用显微切割的方法获取肿瘤组织,然后用PCR的方法以 16号染色体上的 8对引物对 38例鼻咽癌进行分析。结果: 38例鼻咽癌组织中所有标本至少有一个位点出现有杂合性缺失,其中 D165533出现杂合性缺失的频率最高,占 86.1%(31/33),此外,杂合性缺失频率超过50%的有引物 D16S398、D16S390和 D16S420,分别占 78.8%(26/33)、69.4%(25/33)和 57. 6%(19/33)。结论:本研究首次发现在 I6号染色体上存在三个杂合性缺失高频缺失区,分别位于 16p12.3(引物 D16S420)。16q12.2(引物D16S390)和 16q21-22.1(引物D16S533,D16S398)。
Objective: To study the deletion of chromosome 16 in nasopharyngeal carcinoma. Methods: Tumor tissues were obtained by microdissection, and then 38 nasopharyngeal carcinomas were analyzed by PCR with 8 pairs of primers on chromosome 16. Results: There was heterozygous deletion in at least one locus in all 38 specimens of nasopharyngeal carcinoma. The frequency of heterozygosity deletion in D165533 was the highest (86.1%, 31/33). In addition, heterozygosity The primers D16S398, D16S390 and D16S420 with deletion frequencies of over 50% accounted for 78.8% (26/33) and 69.4% (25/33), respectively. 6% (19/33). CONCLUSION: This study is the first to find three heterozygous deletion high frequency deletion regions on chromosome I6, located at 16p12.3 (primer D16S420). 16q12.2 (primers D16S390) and 16q21-22.1 (primers D16S533, D16S398).