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目的:建立复方甘草口服溶液中甘草酸的含量测定方法。方法:采用高效液相色谱法(HPLC),色谱柱为Agilent Eclipse Plus-C18(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(70︰30),检测波长250 nm,柱温25℃,流速1.0 m L/min,进样量20μL。结果:甘草酸浓度在29.18~182.35μg/m L范围内时,样品浓度与其相应色谱峰峰面积积分值呈良好线性关系(r=0.999 2)。精密度、稳定性和重复性试验的相对标准偏差(RSD)分别为0.59%、0.66%和0.40%;样品平均加样回收率为97.47%,RSD为1.49%(n=9)。结论:该方法专属性、重复性和准确性均较好,可用于复方甘草口服溶液中甘草酸的含量测定。
Objective: To establish a method for the determination of glycyrrhizic acid in compound licorice oral solution. Methods: The mobile phase consisted of methanol-0.1% phosphoric acid aqueous solution (70:30) with Agilent Eclipse Plus-C18 column (250 mm × 4.6 mm, 5 μm) using high performance liquid chromatography Column temperature 25 ℃, flow rate 1.0 m L / min, injection volume 20μL. Results: When the concentration of glycyrrhizic acid was in the range of 29.18 ~ 182.35μg / m L, there was a good linear relationship between the sample concentration and the corresponding peak area of the corresponding chromatogram (r = 0.999 2). The relative standard deviations (RSDs) of precision, stability and repeatability were 0.59%, 0.66% and 0.40%, respectively. The average recovery was 97.47% and the RSD was 1.49% (n = 9). Conclusion: The method is specific, reproducible and accurate, and can be used for the determination of glycyrrhizic acid in compound liquorice oral solution.