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[目的]考察生附子醇提物的经口急性毒性。[方法]以70%为溶媒,用索氏提取法制备附子浸提物,经预试验获得正式试验最大给药剂量,设计7个剂量组,组间距1.7倍,给昆明系小鼠灌胃给药一次,剂量为0.04ml(/g·BW),观察7d,用改良寇氏法公式计算半数致死量(LD50)及其95%可信限。[结果]附子醇提物给小鼠经口LD50为230.12mg(/kg·BW),LD50的95%可信限为80.39~658.57mg(/kg·BW)。[结论]生附子醇提物可快速引起小鼠出现急性毒性。
[Objective] To investigate the oral acute toxicity of alcohol extract of Aconite. [Method] With 70% as the solvent, the extract of Aconite was prepared by Soxhlet extraction. The maximum dose of formalin was obtained through the pre-test. Seven dose groups were designed with the group spacing of 1.7 times. The Kunming mice were given gavage Once a dose of 0.04ml (/ g · BW), observed for 7 days, using the modified Cornell formula to calculate the LD50 and its 95% confidence limit. [Result] The oral LD50 of aconite ethanol extract was 230.12 mg (/ kg · BW) and the 95% confidence interval of LD50 was 80.39 ~ 658.57 mg (/ kg · BW). [Conclusion] The alcohol extract of raw monkshood can quickly cause acute toxicity in mice.