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目的 研究旁观者效应在TK/GCV系统杀灭肿瘤细胞中的作用。方法 转TK基因肺癌细胞A549-TK与亲代细胞A549分别以不同比例、高低密度混合培养，加50 (mol/L GCV作用5 d，MTT法测活。结果 当细胞以高密度接种（104/孔），TK+细胞占20%就能观察到较对照细胞出现明显的生长抑制（P<0.05），而细胞以低密度接种（103/孔），TK+细胞占50%出现较对照细胞明显地生长抑制 （P<0.05）。结论 旁观者效应在TK/GCV系统杀灭瘤细胞中起重要作用，且这一效应与细胞间密切接触有关。
Objective To study the role of bystander effect in killing tumor cells by TK / GCV system. Methods The lung cancer cell line A549-TK transfected with TK gene and the parental cell line A549 were cultured in different ratios and high and low density respectively, and treated with 50 (mol / L GCV for 5 days, MTT assay.) Results When cells were inoculated at high density (104 / (P <0.05). When TK + cells accounted for 20%, obvious growth inhibition was observed compared with that of control cells (P <0.05), while cells were inoculated at low density (103 / well) (P <0.05) .Conclusion Bystander effect plays an important role in killing tumor cells by TK / GCV system, and this effect is closely related to the close contact between cells.