论文部分内容阅读
目的:研究脊髓伤害性信息传递中P物质(SP)与N-甲基-D-天冬氨酸(NMDA)受体甘氨酸位点激动剂D-丝氨酸(D-serine)之间的功能联系。方法:在浅麻大鼠,采用行为学方法,测定甩尾反射潜伏期(TFL)并结合鞘内给药途径观察药物作用。结果:鞘内注射D-serine 1000nmol后1.5分钟,TFL明显缩短;在注射D-serine 10nmol前6分钟鞘内施加SP0.05nmol,明显增强D-serine 10nmol引起的TFL缩短效应;选择性NMDA受体甘氨酸位点拮抗剂7-氯犬尿酸1pmol及非选择性PKC抑制剂H-7 10μmol均可阻断这种增强作用。结论:SP可使D-丝氨酸诱发的热痛过敏明显加强,NMDA受体甘氨酸位点及胞内蛋白激酶系统参与了脊髓SP与NMDA受体的相互作用。
OBJECTIVE: To investigate the functional relationship between substance P (SP) and D-serine, a N-methyl-D-aspartate (NMDA) receptor glycine site agonist in spinal nociceptive transmission. Methods: The behavioral methods were used to determine the latency of tail flick (TFL) and the effects of drugs in combination with intrathecal administration in sham rats. Results: After intrathecal injection of 1000nmol D-serine, the TFL was shortened significantly. SP0.05nmol intrathecally intrathecally 6 min before the injection of D-serine 10nmol significantly enhanced the TFL shortening effect induced by D-serine 10nmol. The selective NMDA receptor This potentiation was blocked by 1 pmol of the glycine site antagonist 7-chloro-kynuric acid and 10 μmol of the non-selective PKC inhibitor H-7. CONCLUSION: SP can significantly increase the heat-induced hyperalgesia induced by D-serine. The glycine sites of NMDA receptors and the intracellular protein kinase system are involved in the interaction of SP with NMDA receptors.