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将纳米纤维素分别与高阳离子度的丙烯酸类、乙烯胺类聚合物复合,制备出三维多孔组织工程支架并通过SEM观察了支架的形貌结构,研究了高阳离子度的丙烯酸类、乙烯胺类聚合物的相对分子质量和用量对支架孔隙结构的影响;用图像处理软件对采集的支架截面SEM图进行处理,通过统计计算获得支架孔隙率数据,建立了一种基于SEM图像快速测定支架孔隙率的新方法;最后测定了支架的保水值。结果表明:本文制备的纳米纤维素三维组织工程支架的孔隙率均大于90%。用图像处理得到的孔隙率与用液体置换法测得的孔隙率数值接近,误差低于5%,表明用图像处理方法测定的孔隙率可靠。纳米纤维素三维组织工程支架有很高的保水值(>200%),调整阳离子聚合物的种类和用量可调控其孔隙率和保水值,得到适合组织细胞培养的纳米纤维素三维组织工程支架。
The three-dimensional porous tissue engineering scaffolds were prepared by compounding nanocellulose with high-cationic acrylic and vinyl amine polymers respectively. The morphology and structure of scaffolds were observed by SEM. The effects of high cationic acrylic and vinyl amine The relative molecular mass and the amount of polymer on the scaffold pore structure; using image processing software to collect SEM cross-section of the scaffolds were collected by statistical calculation scaffold porosity data to establish a rapid determination of scaffold porosity based on SEM images The new method; the final determination of the water retention value of the stent. The results show that the porosity of nano-cellulose three-dimensional tissue engineering scaffold prepared in this paper is more than 90%. The porosity obtained by image processing is close to the porosity value measured by the liquid displacement method with an error of less than 5%, indicating that the porosity determined by the image processing method is reliable. Nano-cellulose three-dimensional tissue engineering scaffold has a high water retention (> 200%), adjusting the type and amount of cationic polymer can regulate its porosity and water retention value, to obtain suitable for tissue culture of nano-cellulose three-dimensional tissue engineering scaffold.