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采用聚丙烯酰胺凝胶电泳法,对甘草属( Glycyrrhiza) 植物短荚果系的3 个种和念珠状荚果系6 个种与其近缘属4 个种的子叶和下胚轴进行了超氧化物歧化酶、细胞色素氧化酶和过氧化物同工酶的分析。结果显示:属间、种间具有酶谱差异,每个属有其特征酶谱带。根据同工酶酶谱性状:下胚轴36 个同工酶谱性状和子叶38 个同工酶谱性状,分别对13 个种进行了聚类分析,结果表明,13 个种可划分为2 类;甘草属( Glycyrrhiza) 植物的9 个种很好的聚在一组,盐豆木( H.holodendron) 也在此组,表明两属间关系较近;苦豆子( S.alopecuroides) 、骆驼刺( A.pseudalhagi) 和小花棘豆( O.glabra) 在另一组,它们关系较近。
Polyacrylamide gel electrophoresis was performed on three species of Brassica juncea and six species of rosary pod and four cotyledons of hypocotyls and hypocotyls of the genus Glycyrrhiza for superoxide dismutase Analysis of Enzymes, Cytochrome Oxidases and Peroxidase Isoenzymes. The results showed that there was a difference in zymogram between genus and species, and each genus had its characteristic zymogram. According to the isozyme zymogram: Thirty-six isozyme traits in hypocotyls and 38 isozyme traits in cotyledons, 13 species were clustered respectively. The results showed that 13 species could be divided into two groups Nine species of Glycyrrhiza plants clustered well together, and H. holodendron was also in this group, indicating that the two genera were closely related to each other. S.alopecuroides, (A. pseudalhagi) and O.glabra in the other group, they are closely related.