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用ＰＣＲ方法克隆了中国丙型肝炎病毒（ＨＣＶ）感染者的５＇端非编码区（５＇－ｎｏｎｃｏｄｉｎｇｒｅｇｉｏｎ，５＇－ＮＣＲ）序列，并根据此序列合成引物检测我国ＨＣＶ各高危感染人群的病毒复制情况，结果显示ＨＣＶＲＮＡ在慢性丙型肝炎患者的血清标本中的阳性率为８１．８％（９／１１），在ＨＣＶ抗体阳性的血液透析患者血清标本中的阳性率为６３．６％（７／１１），在ＨＣＶ抗体阳性的献血员血清标本中的阳性率为２０％（４／２０），在ＨＣＶ抗体阳性的肝细胞癌标本中的阳性率为１００％（５／５），在ＨＣＶ抗体阴性的肝细胞癌标本中的阳性率为３３．３％（７／２１）。表明ＨＣＶ的体内复制情况在各高危风险人群中的情况有明显差别；提示ＨＣＶ感染也是我国肝细胞癌发生的重要风险因子之一。
The 5’-noncoding region (5’-NCR) sequence was cloned by PCR from hepatitis C virus (HCV) infected individuals in China. The primers were then used to detect the HCV The results showed that the positive rate of HCVRNA in serum samples of patients with chronic hepatitis C was 81.8% (9/11), and the positive rate of HCV RNA in serum samples of HCV antibody-positive hemodialysis patients was 63.6% ( 7/11). The positive rate was 20% (4/20) in serum samples of HCV antibody-positive donors and 100% (5/5) in HCV antibody-positive hepatocellular carcinoma specimens. The positive rate of HCV antibody-negative hepatocellular carcinoma specimens was 33.3% (7/21). Indicating that in vivo replication of HCV in the high-risk groups have significant differences in the situation; suggesting that HCV infection is also one of the important risk factors for hepatocellular carcinoma in our country.