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目的 探讨Bel-7402人肝癌细胞株分化的生化指标及全反式维甲酸(RA)和丁酸钠(SB)联合作用对Bel-7402人肝癌细胞株的分化作用。方法 以反映肝癌特异性的γ-谷氨酰转肽酶(γ-GT)及反映肝癌细胞分化的酪氨酸α酮戊二酸转氨酶(TAT)和碱性磷酸酶(ALP)为指标,通过Huseby法测定处理前后肝癌细胞γ-GT活性的变化,Diamondstone分光光度法测定TAT活性,而ALP活性的测定则用Bowers法。结果 RA、SB及两者联合作用均能使Bel-7402人肝癌细胞株γ-GT和耐热型ALP活性降低,而TAT和ALP活性则升高。结论 RA、SB及两者联合作用均能有效地诱导Bel-7402人肝癌细胞株分化,联合作用效果优于单独作用,RA、SB对肝癌细胞的诱导分化作用可能只通过改变相关酶的基因表达
Objective To investigate the biochemical markers of Bel-7402 human hepatoma cell line differentiation and the effect of combined action of all-trans retinoic acid (RA) and sodium butyrate (SB) on Bel-7402 human hepatoma cell line. Methods γ-GT and γ-GT reflecting tyrosine α-ketoglutarate transaminase (TAT) and alkaline phosphatase (ALP) The Huseby method was used to determine the changes of γ-GT activity in hepatocellular carcinoma cells before and after treatment. The activity of TAT was determined by Diamondstone spectrophotometry. The activity of ALP was determined by Bowers method. Results The combination of RA, SB and both could decrease the activity of γ-GT and heat-tolerant ALP of Bel-7402 human hepatoma cell line, while the activity of TAT and ALP increased. Conclusion The combination of RA, SB and both can effectively induce Bel-7402 human hepatocellular carcinoma cell line differentiation, the combined effect is better than alone, RA, SB on the induction of differentiation of liver cancer cells may only by changing the gene expression of related enzymes