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分别以自交系M17和77的姊妹系,姊妹种及改良单交种为材料,通过聚丙烯酰胺凝胶胶电泳(PAGE),分析了各材料幼苗的过氧化物酶同工酶和酯酶同工酶,并采用SDS-PAGE,分析了盐溶蛋白、醇溶蛋白和谷蛋白。结果表明:姊妹系间,姊妹种与姊姊系间的酯酶同工酶谱和三种蛋白图谱基本相同,过氧化物酶同工酶谱则存在一定差异;姊姊种均无“杂种酶带”出现,这与亲缘较远的两自交系组配的杂交种(单交种)具有本质差别;改良单交种与对应单交种的两种同工酶谱和蛋白图谱则相同。
By using polyacrylamide gel electrophoresis (PAGE), the sister lines, sister lines and modified single cross of inbred lines M17 and 77 were used as materials to analyze the peroxidase isoenzyme and esterase Isozymes, and analyzed by SDS-PAGE salt protein, gliadin and gluten. The results showed that there were some differences in the patterns of esterase isozyme and three protein among sister lines, sister lines and sister lines, while there were some differences in the isozymes of peroxidase. None of the elder sister strains had hybrid enzymes Band ", which is essentially different from the hybrids (single cross) that are mated with two distantly related inbred lines. The two isozymes and protein profiles of the improved single cross and the corresponding single cross are the same.