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目的比较2种方法检测人类免疫缺陷病毒(HIV)1型病毒载量的相关性及一致性,为合理评价HIV/AIDS病程进展及抗HIV药物疗效提供参考。方法对四川省凉山州95例在治HIV/AIDS的血浆样本同时用分支DNA杂交实验(bDNA)法和核酸序列扩增实验(NASBA)法检测HIV-1病毒载量,结果进行比较分析。结果该2种方法测得阳性样本均值bDNA法为3.75±1.05 log copies/mL,NASBA法为4.22±1.23 log copies/mL,差异有统计学意义(t=6.09,P<0.05);2种方法测出的病毒载量呈显著直线相关(r=0.852,P<0.01);2种方法测出的病毒载量阳性率差异无统计学意义(χ2=0.836,P>0.05)。结论 bDNA法和NASBA法检测HIV-1病毒载量具有高度的一致性,在实际工作中均可选用,但是两者检测结果的数值不能完全等同。建议抗HIV药物疗效评价最好应用同一种方法的检测结果,使其治疗前后的结果具有可比性和一致性。
Objective To compare the correlation and consistency between two methods for detecting HIV - 1 viral load and provide a reference for evaluating the progress of HIV / AIDS and the efficacy of anti - HIV drugs. Methods HIV-1 viral load was detected in 95 cases of HIV / AIDS-treated plasma samples from Liangshan Prefecture in Sichuan Province by the method of bDNA and NASBA. Results The positive rate of bax method was 3.75 ± 1.05 log copies / mL, and that of NASBA method was 4.22 ± 1.23 log copies / mL, the difference was statistically significant (t = 6.09, P <0.05) (R = 0.852, P <0.01). There was no significant difference in the positive rates of viral load between the two methods (χ2 = 0.836, P> 0.05). Conclusion The results of bDNA and NASBA assays showed that HIV-1 viral load was highly consistent and could be used in practice. However, the values of both test results could not be completely equal. Proposed anti-HIV drug efficacy evaluation is best to use the same method of test results, the results before and after treatment to be comparable and consistent.