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目的研究雷帕霉素对急性 T 细胞白血病细胞株 Jur-kat 的作用及其机制,并探讨其对端粒酶活性的影响,预测雷帕霉素参与急性 T 细胞白血病治疗方案的可行性。方法应用 MTT 法检测雷帕霉素对 Jurkat 细胞增殖的影响;Annexin V-P1双染法流式细胞仪检测其对细胞凋亡的影响;PI 染色 DNA 含量分析流式细胞仪检测其对细胞周期
Objective To investigate the effect of rapamycin on Jur-kat cell line and its mechanism, and to explore the impact of rapamycin on telomerase activity and predict the feasibility of rapamycin in the treatment of acute T cell leukemia. Methods The effect of rapamycin on the proliferation of Jurkat cells was detected by MTT assay. The effect of rapamycin on the apoptosis of Jurkat cells was detected by flow cytometry with Annexin V-P1 double staining. The content of DNA of PI staining was analyzed by flow cytometry.