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采用Fura－2／AM荧光探剂法测定不同龄大鼠大脑皮层和海马两个脑区细胞内游离钙浓度（[Ca2+]i）以及尼莫地平对老龄大鼠（24月龄）上述两个脑区[Ca2+]i的影响，另外用同位素标记法测定尼莫地平对老龄大鼠上述两脑区细胞线粒体摄钙的影响。结果发现，随增龄两个脑区细胞[Ca2+]i呈渐进性增长，应用尼莫地平后．老龄大鼠两脑区细胞[Ca2＋]i的升高明显被抑制（P＜0．01），同时细胞线粒体摄钙能力亦明显升高（P＜0.01）但无明显剂量依赖性。提示尼莫地平可通过阻断钙通道、促进细胞内钙库对Ca2＋封存等不同途径降低神经细胞[Ca2＋]i，延缓神经细胞的损伤和老化。
Fura-2 / AM fluorescence probe method was used to determine the intracellular free calcium concentration ([Ca2 +] i) in the cerebral cortex and hippocampus in different age groups and the effects of nimodipine on the two above-mentioned old rats (24 months old) Brain [Ca2 +] i, and the effect of nimodipine on mitochondrial calcium uptake in the above two brain regions of aged rats was also measured by isotope labeling. The results showed that with the age of two brain cells [Ca2] i gradually increased, the application of nimodipine. The elevation of [Ca2 +] i in both brain regions was significantly inhibited in old rats (P <0.01), and the mitochondrial calcium uptake capacity was also significantly increased (P <0.01) but not in a dose-dependent manner. These results suggest that nimodipine can decrease the [Ca2 +] i of nerve cells by blocking the calcium channels and promoting the Ca2 + sequestration of intracellular calcium stores, which can delay the damage and aging of nerve cells.